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EZH2 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - EZH2 Antibody AW5436
    All lanes : Anti-EZH2 Antibody at 1:1000 dilution Lane 1: CEM whole cell lysates Lane 2: mouse spleen lysates Lane 3: MDA-MB468 whole cell lysates Lane 4: T47D whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q15910
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=85,86,81,84,80;M=85,81 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 2146
Other Names Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, EZH2, KMT6
Dilution WB~~1:1000
Target/Specificity This EZH2 antibody is generated from rabbits immunized with a recombinant protein from human EZH2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEZH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EZH2
Synonyms KMT6
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Cellular Location Nucleus
Tissue Location Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Research Areas
Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.
Author : Hou TY1,2,3,Chen MR4,Chou YC5,Kan PC4,Tsai YT1,6,Cha TL1,4,6.
Front Immunol. 2017 Jul 10;8:790. doi: 10.3389/fimmu.2017.00790. eCollection 2017.
28740493

BACKGROUND

EZH2 is a protein that transfers sulfate to the C-4 hydroxyl of beta-1,4-linked GalNAc flanked by GlcUA residues in chondroitin.

REFERENCES

Kang,H.G., et.al., J. Biol. Chem. 277 (38), 34766-34772 (2002)
Hiraoka,N., et.al., J. Biol. Chem. 275 (26), 20188-20196 (2000)

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