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GNL3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - GNL3 Antibody (N-term) AW5435
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling GNL3 with AW5435 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing both nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).
  • 1 - GNL3 Antibody (N-term) AW5435
    All lanes : Anti-GNL3 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: NCCIT whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF
Primary Accession Q9BVP2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=62, 61 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 26354
Antigen Region 91-124
Other Names Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS
Dilution IF~~1:25
WB~~1:1000
Target/Specificity This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-124 amino acids from the N-terminal region of human GNL3.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNL3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNL3
Synonyms E2IG3, NS
Function May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
Cellular Location Nucleus. Nucleus, nucleolus. Note=Shuttles between the nucleus and nucleolus
Tissue Location Increased levels in lung tissue in cancer patients.
Research Areas

BACKGROUND

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

REFERENCES

Charpentier A.H.,et al.Cancer Res. 60:5977-5983(2000).
Han C.,et al.Int. J. Mol. Med. 16:205-213(2005).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Andersen J.S.,et al.Curr. Biol. 12:1-11(2002).

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