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Mouse Klf4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - Mouse Klf4 Antibody (Center) AW5431
    Overlay histogram showing NIH/3T3 cells stained with AW5431 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5431, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - Mouse Klf4 Antibody (Center) AW5431
    All lanes : Anti-Klf4 Antibody (Center) at 1:1000 dilution Lane 1: mouse heart lysates Lane 2: mouse small intestine lysates Lane 3: mouse testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC
Primary Accession Q60793
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW M=52 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 16600
Antigen Region 321-354
Other Names Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, Klf4, Ezf, Gklf, Zie
Dilution FC~~1:25
WB~~1:1000
Target/Specificity This Mouse Klf4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 321-354 amino acids from the Central region of mouse Klf4.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Klf4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Klf4
Synonyms Ezf, Gklf, Zie
Function Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).
Cellular Location Nucleus.
Tissue Location Highest expression in the colon. Lower levels in testis, lung and small intestine
Research Areas

BACKGROUND

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).

REFERENCES

Shields J.M.,et al.J. Biol. Chem. 271:20009-20017(1996).
Garrett-Sinha L.A.,et al.J. Biol. Chem. 271:31384-31390(1996).
Mahatan C.S.,et al.Nucleic Acids Res. 27:4562-4569(1999).
Chen Z.-Y.,et al.Exp. Cell Res. 281:19-27(2002).
Carninci P.,et al.Science 309:1559-1563(2005).

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