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DNASE1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DNASE1 Antibody (Center) AW5430
    All lanes : Anti-DNASE1 Antibody (Center) at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P24855
Other Accession P00639
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=31;M=32 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 1773
Antigen Region 87-121
Other Names Deoxyribonuclease-1, Deoxyribonuclease I, DNase I, Dornase alfa, DNASE1, DNL1, DRNI
Dilution WB~~1:1000
Target/Specificity This DNASE1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 87-121 amino acids from the Central region of human DNASE1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNASE1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNASE1
Synonyms DNL1, DRNI
Function Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).
Cellular Location Secreted. Nucleus envelope. Note=Secretory protein, stored in zymogen granules and found in the nuclear envelope
Tissue Location Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva
Research Areas

BACKGROUND

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).

REFERENCES

Shak S.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:9188-9192(1990).
Yasuda T.,et al.Ann. Hum. Genet. 59:1-15(1995).
Oliveri M.,et al.Eur. J. Immunol. 31:743-751(2001).
Kominato Y.,et al.FEBS J. 273:3094-3105(2006).
Martin J.,et al.Nature 432:988-994(2004).

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