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(Mouse) Msi1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - (Mouse) Msi1 Antibody (Center) AW5419
    All lanes : Anti-Msi1 Antibody (Center) at 1:1000 dilution Lane 1: Neuro-2a whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q61474
Other Accession Q8K3P4, O43347
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Calculated MW M=39, 34;H=39;R=39 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 17690
Antigen Region 81-113
Other Names RNA-binding protein Musashi homolog 1, Musashi-1, Msi1, Msi1h
Dilution WB~~1:1000
Target/Specificity This mouse Msi1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 81-113 amino acids from the Central region of mouse Msi1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Msi1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Msi1
Synonyms Msi1h
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Cellular Location Cytoplasm. Nucleus
Tissue Location Detected in olfactory bulb, brain stem, small intestine, and at low levels in brain cortex, hippocampus and ovary. Detected in neural progenitor cells, including neural stem cells.
Research Areas

BACKGROUND

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

REFERENCES

Sakakibara S.,et al.Dev. Biol. 176:230-242(1996).
Carninci P.,et al.Science 309:1559-1563(2005).
Imai T.,et al.Mol. Cell. Biol. 21:3888-3900(2001).
Sakakibara S.,et al.Proc. Natl. Acad. Sci. U.S.A. 99:15194-15199(2002).
Nagata T.,et al.J. Mol. Biol. 287:315-330(1999).

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