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RAB35 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - RAB35 Antibody (C-term) AW5404
    All lanes : Anti-RAB35 Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysates Lane 2: Hela whole cell lysates Lane 3: HepG2 whole cell lysates Lane 4: mouse brain lysates Lane 5: NIH/3T3 whole cell lysates Lane 6: THP-1 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - RAB35 Antibody (C-term) AW5404
    Immunohistochemical analysis of paraffin-embedded H.small intestine section using RAB35 Antibody (C-term)(Cat#AW5404). AW5404 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession Q15286
Other Accession Q5U316, Q6PHN9
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=23;M=23;R=23 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 11021
Antigen Region 166-199
Other Names Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This RAB35 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 166-199 amino acids from the C-terminal region of human RAB35.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB35 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB35
Synonyms RAB1C, RAY
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane, clathrin- coated pit. Cytoplasmic vesicle, clathrin-coated vesicle Endosome. Melanosome. Note=Present on sorting endosomes and recycling endosome tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified in stage I to stage IV melanosomes
Research Areas

BACKGROUND

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth.

REFERENCES

Zhu A.X.,et al.Biochem. Biophys. Res. Commun. 205:1875-1882(1994).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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