|Application ||IF, IHC-P|
|Calculated MW||H=55 M=55 KDa|
|Other Names||Histone deacetylase 2, HD2, HDAC2|
|Target/Specificity||This HDAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-488 amino acids from the C-terminal region of human HDAC2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HDAC2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Widely expressed; lower levels in brain and lung|
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
Choi, Y.B., et al., J. Biol. Chem. 279(49):50930-50941 (2004).
Zhu, P., et al., Cancer Cell 5(5):455-463 (2004).
Longworth, M.S., et al., J. Virol. 78(7):3533-3541 (2004).
Lu, Y., et al., J. Biol. Chem. 278(48):47792-47802 (2003).
Verdin, E., et al., Trends Genet. 19(5):286-293 (2003).