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>   首页   >   产品   >   一抗   >   细胞生物学   >   CF150 Antibody (Center)   

CF150 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - CF150 Antibody  (Center) AW5397
    Overlay histogram showing U-2OS cells stained with AW5397 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5397, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - CF150 Antibody  (Center) AW5397
    All lanes : Anti-CF150 Antibody (Center) at 1:1000 dilution Lane 1: human cerebellum lysates Lane 2: human spleen lysates Lane 3: HT-29 whole cell lysates Lane 4: MCF-7 whole cell lysates Lane 5: THP-1 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q8N884
Other Accession NP_612450.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=59,50 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 115004
Antigen Region 266-295
Other Names Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, Mab-21 domain-containing protein 1, MB21D1, C6orf150
Dilution FC~~1:25
WB~~1:1000
Target/Specificity This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCF150 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MB21D1
Synonyms C6orf150
Function Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
Cellular Location Cytoplasm, cytosol
Tissue Location Expressed in the monocytic cell line THP1.
Research Areas

BACKGROUND

The exact function of C6orf150 remains unknown.

REFERENCES

Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)

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