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ACTN2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ACTN2 Antibody (Center) AW5383
    All lanes : Anti-ACTN2 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: A431 whole cell lysates Lane 4: human skeletal muscle lysates Lane 5: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P35609
Other Accession Q9JI91, P20111, Q3ZC55
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=104;M=104 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 88
Antigen Region 352-386
Other Names Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2
Dilution WB~~1:1000
Target/Specificity This ACTN2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 352-386 amino acids from the Central region of human ACTN2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTN2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTN2
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Cellular Location Cytoplasm, myofibril, sarcomere, Z line Note=Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle
Tissue Location Expressed in both skeletal and cardiac muscle.
Research Areas

BACKGROUND

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

REFERENCES

Beggs A.H.,et al.J. Biol. Chem. 267:9281-9288(1992).
Tiso N.,et al.Biochem. Biophys. Res. Commun. 265:256-259(1999).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Faulkner G.,et al.J. Cell Biol. 146:465-475(1999).

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