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SLC25A37 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SLC25A37 Antibody (C-term) AW5376
    All lanes : Anti-SLC25A37 Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysates Lane 2: rat spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - SLC25A37 Antibody (C-term) AW5376
    SLC25A37 Antibody (C-term) (Cat. #AW5376) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC25A37 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - SLC25A37 Antibody (C-term) AW5376
    SLC25A37 Antibody (C-term) (Cat. #AW5376) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - SLC25A37 Antibody (C-term) AW5376
    Immunofluorescent analysis of Hela cells, using SLC25A37 Antibody (C-term) (Cat. #AW5376). AW5376 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, IHC-P, WB
Primary Accession Q9NYZ2
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=37,21;M=38;R=37 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 51312
Antigen Region 309-338
Other Names Mitoferrin-1, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein, Solute carrier family 25 member 37, SLC25A37, MFRN, MSCP
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
IF~~1:25
Target/Specificity This SLC25A37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human SLC25A37.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC25A37 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC25A37
Synonyms MFRN, MSCP
Function Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme (By similarity).
Cellular Location Mitochondrion inner membrane; Multi-pass membrane protein
Research Areas

BACKGROUND

SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al., 2006 [PubMed 16949250]).[supplied by OMIM].

REFERENCES

Kaneko, K., et al. FEBS J. 276(5):1370-1382(2009) Haitina, T., et al. Genomics 88(6):779-790(2006) Shaw, G.C., et al. Nature 440(7080):96-100(2006) Li, Q.Z., et al. Mamm. Genome 12(11):830-836(2001)

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