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(Mouse) Shh Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - (Mouse) Shh Antibody (C-term) AW5359
    All lanes : Anti-Shh Antibody (C-term)(AW5359) at 1/1000 dilution Lane 1: mouse liver lysates Lane 2: mouse lung lysates Lane 3: mouse stomach lysates Lane 4: rat lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - (Mouse) Shh Antibody (C-term) AW5359
    Immunohistochemical analysis of paraffin-embedded M. lung section using (Mouse) Shh Antibody (C-term)(Cat#AW5359). AW5359 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession Q62226
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW M=48;Rat=48 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 20423
Antigen Region 397-431
Other Names Sonic hedgehog protein, SHH, HHG-1, Sonic hedgehog protein N-product, Sonic hedgehog protein C-product, Shh, Hhg1
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This (Mouse) Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 397-431 amino acids from the C-terminal region of Mouse Shh.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Shh Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Shh
Synonyms Hhg1
Function Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO.
Cellular Location Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell
Tissue Location Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina
Research Areas

BACKGROUND

Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity).

REFERENCES

Echelard Y.,et al.Cell 75:1417-1430(1993).
McMahon A.P.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Chang D.T.,et al.Development 120:3339-3353(1994).
Carninci P.,et al.Science 309:1559-1563(2005).
Roelink H.,et al.Cell 81:445-455(1995).

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