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AGR2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - AGR2 Antibody (N-term) AW5351
    All lanes : Anti-AGR2 Antibody (N-term)(AW5351) at 1/1000 dilution Lane 1: HT-29 whole cell lysates Lane 2: T47D whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - AGR2 Antibody (N-term) AW5351
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (Cat.#AW5351), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - AGR2 Antibody (N-term) AW5351
    Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term)(Cat#AW5351).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P
Primary Accession O95994
Reactivity Human
Predicted Mouse, Zebrafish
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Clone Names RB14041
Calculated MW H=20;M=20;Z=20 KDa
Additional info
Gene ID 10551
Other Names AGR2; AG2; Anterior gradient protein 2 homolog; HPC8; Secreted cement gland protein XAG-2 homolog
Target/Specificity This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAGR2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AGR2
Synonyms AG2
Function Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Cellular Location Secreted. Endoplasmic reticulum
Tissue Location Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
Research Areas

BACKGROUND

Anterior gradient 2 (AGR2) is known as a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. AGR2 has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells (CTCs). Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells, but conditions leading to increased expression of AGR2 are not well understood.

REFERENCES

Zweitzig,D.R., Mol. Cell. Biochem. 306 (1-2), 255-260 (2007)
Zhang,Y., Prostate Cancer Prostatic Dis. 10 (3), 293-300 (2007)
Fletcher,G.C., Br. J. Cancer 88 (4), 579-585 (2003)

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