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NCAM1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NCAM1 Antibody (C-term) AW5350
    Western blot analysis of lysates from HT-1080 cell line,human brain,human cerebellum tissue lysate(from left to right), using NCAM1 Antibody (C-term)(Cat. #AW5350). AW5350 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 4 - NCAM1 Antibody (C-term) AW5350
    Flow cytometric analysis of SH-SY5Y cells using NCAM1 Antibody (C-term)(green, Cat#AW5350) compared to an isotype control of rabbit IgG(blue). AW5350 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB
Primary Accession P13591
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=95,93 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4684
Antigen Region 829-863
Other Names Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56, NCAM1, NCAM
Dilution WB~~1:1000
FC~~1:25
Target/Specificity This NCAM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 829-863 amino acids from the C-terminal region of human NCAM1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCAM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCAM1
Synonyms NCAM
Function This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Cell membrane; Lipid-anchor, GPI- anchor Isoform 5: Secreted.
Research Areas

BACKGROUND

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

REFERENCES

Barton C.H.,et al.Development 104:165-173(1988).
Lanier L.L.,et al.J. Immunol. 146:4421-4426(1991).
Saito S.,et al.Lung Cancer 10:307-318(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.

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