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AFAP1-Y451 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - AFAP1-Y451 Antibody AW5348
    Western blot analysis of lysates from Hela,MCF-7,MDA-MB-231 cell line (from left to right), using Phospho-AFAP1-Y451.ctrl(Cat. #AW5348). AW5348 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q8N556
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=81,90;M=81;Rat=81 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 60312
Antigen Region 444-477
Other Names Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP
Dilution WB~~1:1000
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 444-477 amino acids from human.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAFAP1-Y451 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AFAP1
Synonyms AFAP
Function Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction
Tissue Location Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF- 7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
Research Areas

BACKGROUND

Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.

REFERENCES

Han B.,et al.J. Biol. Chem. 279:54793-54801(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Olsen J.V.,et al.Cell 127:635-648(2006).

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