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TCL1A Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - TCL1A Antibody (Center) AW5346
    Western blot analysis of lysates from Ramos,Raji,Daudi cell line (from left to right), using TCL1A Antibody (Center)(Cat. #AW5346). AW5346 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 4 - TCL1A Antibody (Center) AW5346
    Flow cytometric analysis of Ramos cells using TCL1A Antibody (Center)(green, Cat#AW5346) compared to an isotype control of rabbit IgG(blue). AW5346 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB
Primary Accession P56279
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=13 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 8115
Antigen Region 32-66
Other Names T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1, Oncogene TCL1, Protein p14 TCL1, TCL1A, TCL1
Dilution WB~~1:1000
FC~~1:25
Target/Specificity This TCL1A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-66 amino acids from the Central region of human TCL1A.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCL1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCL1A
Synonyms TCL1
Function Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
Cellular Location Cytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Note=Microsomal fraction
Tissue Location Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation
Research Areas

BACKGROUND

Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.

REFERENCES

Virgilio L.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:12530-12534(1994).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Fu T.-B.,et al.Cancer Res. 54:6297-6301(1994).
Laine J.,et al.Mol. Cell 6:395-407(2000).
Pekarsky Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:3028-3033(2000).

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