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ALDH2 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ALDH2 Antibody AW5333
    Western blot analysis of lysates from HepG2,A549 cell line,mouse liver,mouse lung,rat liver tissue lysate(from left to right), using ALDH2 Antibody(Cat. #AW5333). AW5333 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - ALDH2 Antibody AW5333
    Formalin-fixed and paraffin-embedded human brain tissue reacted with ALDH2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ALDH2 Antibody AW5333
    ALDH2 Antibody (Cat. #AW5333) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P05091
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=56;M=57;Rat=56 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 217
Other Names ALDH2; ALDM; Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This ALDH2 antibody is generated from rabbits immunized with human ALDH2 recombinant protein.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH2
Synonyms ALDM
Cellular Location Mitochondrion matrix.
Research Areas

BACKGROUND

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.

REFERENCES

Guo,Y.M., World J. Gastroenterol. 14 (9), 1444-1449 (2008)
Chen,L., PLoS Med. 5 (3), E52 (2008)
Teeguarden,J.G., Inhal Toxicol 20 (4), 375-390 (2008)
Yoshida,A., Pharmacogenetics 2 (4), 139-147 (1992)

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