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LRP12 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - LRP12 Antibody (C-term) AW5323
    Western blot analysis of lysates from human heart,human skeletal muscle tissue lysate (from left to right), using LRP12 Antibody (C-term)(Cat. #AW5323). AW5323 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - LRP12 Antibody (C-term) AW5323
    LRP12 Antibody (C-term) (Cat. #AW5323)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRP12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 3 - LRP12 Antibody (C-term) AW5323
    Confocal immunofluorescent analysis of LRP12 Antibody (C-term)(Cat#AW5323) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 4 - LRP12 Antibody (C-term) AW5323
    LRP12 Antibody (C-term) (Cat. #AW5323) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IF, IHC-P, WB
Primary Accession Q9Y561
Other Accession Q8BUJ9, Q9BE74, NP_038465.1
Reactivity Human
Predicted Mouse, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=95,93;M=95,93 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 29967
Antigen Region 635-662
Other Names LRP12; ST7; Low-density lipoprotein receptor-related protein 12; Suppressor of tumorigenicity 7 protein
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
Target/Specificity This LRP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 635-662 amino acids from the C-terminal region of human LRP12.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLRP12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LRP12
Synonyms ST7
Function Probable receptor, which may be involved in the internalization of lipophilic molecules and/or signal transduction. May act as a tumor suppressor.
Cellular Location Membrane; Single-pass type I membrane protein Membrane, coated pit
Tissue Location Widely expressed in heart, skeletal muscle, brain, lung, placenta and pancreas, but not in tissues consisting of a large number of epithelial cells, such as liver and kidney Expressed at very low levels in a number of tumor-derived cell lines.
Research Areas

BACKGROUND

This gene encodes a member of the low-density lipoprotein receptor related protein family. The product of this gene is a transmembrane protein that is differentially expressed in many cancer cells. Alternate splicing results in multiple transcript variants.

REFERENCES

Garnis, C., et al. Oncogene 23(14):2582-2586(2004)
Battle, M.A., et al. Biochemistry 42(24):7270-7282(2003)
Qing, J., et al. Oncogene 18(2):335-342(1999)

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