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PNP Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PNP Antibody (Center) AW5316
    Western blot analysis of lysates from Daudi,HT-1080,K562,MCF-7,MDA-MB231 cell line (from left to right), using PNP Antibody (Center)(Cat. #AW5316). AW5316 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P00491
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW H=32;M=32;Rat=32 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4860
Antigen Region 145-178
Other Names Purine nucleoside phosphorylase, PNP, Inosine phosphorylase, Inosine-guanosine phosphorylase, PNP, NP
Dilution WB~~1:1000
Target/Specificity This PNP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 145-178 amino acids from the Central region of human PNP.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPNP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PNP
Synonyms NP
Function The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm
Tissue Location Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology
Research Areas

BACKGROUND

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.

REFERENCES

Williams S.R.,et al.Nucleic Acids Res. 12:5779-5787(1984).
Williams S.R.,et al.J. Biol. Chem. 262:2332-2338(1987).
Yu L.,et al.Environ. Health Perspect. 111:1421-1427(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.

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