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TIMM50 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - TIMM50 Antibody (N-term) AW5314
    Western blot analysis of lysates from Hela,HepG2,SH-SY5Y cell line (from left to right), using TIMM50 Antibody (N-term)(Cat. #AW5314). AW5314 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q3ZCQ8
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Clone Names RB49868
Calculated MW H=40,50 KDa
Additional info
Gene ID 92609
Other Names Mitochondrial import inner membrane translocase subunit TIM50, TIMM50, TIM50
Target/Specificity This TIMM50 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-56 amino acids from the N-terminal region of human TIMM50.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTIMM50 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TIMM50
Synonyms TIM50
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein
Tissue Location Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level)
Research Areas

BACKGROUND

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.

REFERENCES

Guo Y.,et al.J. Biol. Chem. 279:24813-24825(2004).
Zheng H.,et al.Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Zhang C.,et al.Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Bienvenut W.V.,et al.Submitted (JUN-2005) to UniProtKB.

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