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SNCA Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - SNCA Antibody (C-term) AW5309
    AW5309 staining SNCA in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - SNCA Antibody (C-term) AW5309
    Western blot analysis of lysates from human brain,mouse brain tissue lysate (from left to right), using SNCA Antibody (C-term)(Cat. #AW5309). AW5309 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 4 - SNCA Antibody (C-term) AW5309
    Flow cytometric analysis of HeLa cells using SNCA Antibody (C-term)(green, Cat#AW5309) compared to an isotype control of rabbit IgG(blue). AW5309 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, IHC-P
Primary Accession P37840
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=14,13;M=14;Rat=15 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 6622
Antigen Region 92-125
Other Names Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
Dilution IHC-P~~1:25
WB~~1:1000
FC~~1:25
Target/Specificity This SNCA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-125 amino acids from the C-terminal region of human SNCA.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNCA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNCA
Synonyms NACP, PARK1
Function Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein- alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Cellular Location Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Secreted. Note=Membrane-bound in dopaminergic neurons.
Tissue Location Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain
Research Areas

BACKGROUND

May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

REFERENCES

Ueda K.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:11282-11286(1993).
Campion D.,et al.Genomics 26:254-257(1995).
Ueda K.,et al.Biochem. Biophys. Res. Commun. 205:1366-1372(1994).
Xia Y.,et al.Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
Touchman J.W.,et al.Genome Res. 11:78-86(2001).

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