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NDUA4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NDUA4 Antibody (C-term) AW5303
    Western blot analysis of lysates from Hela,HL-60,SW480 cell line (from left to right), using NDUA4 Antibody (C-term)(Cat. #AW5303). AW5303 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 3 - NDUA4 Antibody (C-term) AW5303
    Fluorescent image of Hela cells stained with NDUA4 Antibody (C-term)(Cat#AW5303). AW5303 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - NDUA4 Antibody (C-term) AW5303
    Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUA4 Antibody (C-term)(Cat#AW5303). AW5303 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF
Primary Accession O00483
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW H=9;M=9 KDa
Additional info
Gene ID 4697
Other Names NDUFA4;NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4
Target/Specificity This NDUA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-80 amino acids from the C-terminal region of human NDUA4.
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFA4
Function Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance.
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
Research Areas

BACKGROUND

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

REFERENCES

Kim J.W., et al. Biochem. Mol. Biol. Int. 43:669-675(1997).
Kanagarajah D., et al. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Ebert L., et al. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Halleck A., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W., et al. Science 300:767-772(2003).

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