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NRAS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NRAS Antibody (C-term) AW5296
    Western blot analysis of lysates from MCF-7,A375 cell line (from left to right), using NRAS Antibody (C-term)(Cat. #AW5296). AW5296 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - NRAS Antibody (C-term) AW5296
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - NRAS Antibody (C-term) AW5296
    NRAS Antibody (C-term) (Cat. #AW5296) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P
Primary Accession P01111
Other Accession Q2MJK3
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB14461
Calculated MW H=21;M=21;Rat=21 KDa
Additional info
Gene ID 4893
Other Names NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras
Target/Specificity This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-179 amino acids from the C-terminal region of human NRAS.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRAS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRAS
Synonyms HRAS1
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus
Research Areas

BACKGROUND

NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

REFERENCES

Smalley,K.S., Cancer Res. 68 (14), 5743-5752 (2008)
Banerji,U., Mol. Cancer Ther. 7 (4), 737-739 (2008)

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