注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   神经科学   >   TBP Antibody   

TBP Antibody

Mouse Monoclonal Antibody (Mab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - TBP Antibody AW5294
    Western blot analysis of lysates from Hela,HepG2,mouse NIH/3T3,Jurkat cell line and human testis tissue (from left to right), using TBP Antibody (Cat. #AW5294). AW5294 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - TBP Antibody AW5294
    Immunohistochemical analysis of paraffin-embedded H. testis section using TBP Antibody(Cat#AW5294). AW5294 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 产品详情
  • 实验流程
  • 背景知识
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P20226
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Calculated MW H=38;M=35 KDa
Isotype IgG1
Antigen Source HUMAN
Additional Information
Gene ID 6908
Antigen Region 1-308
Other Names TBP;GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA-box-binding protein; TATA sequence-binding protein; TATA-box-binding protein; TATA-binding factor; TATA-box-binding protein; TATA-box factor; TATA-box-binding protein; Transcription initiation factor TFIID TBP subunit
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity Purified His-tagged TBP protein was used to produced this monoclonal antibody.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBP
Synonyms GTF2D1, TF2D, TFIID
Function General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Cellular Location Nucleus.
Tissue Location Widely expressed, with levels highest in the testis and ovary.
Research Areas

BACKGROUND

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

REFERENCES

Hoffmann A., et al. Nature 346:387-390(1990).
Peterson M.G., et al. Science 248:1625-1630(1990).
Kao C.C., et al. Science 248:1646-1650(1990).
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Mungall A.J., et al. Nature 425:805-811(2003).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69369083 tech.abgent@wuxiapptec.com.


我要评价