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Histone H3 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - Histone H3 Antibody AW5292
    All lanes : Anti-Histone H3 Antibody at 1:3000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: C2C12 whole cell lysate Lane 7: PC-12 whole cell lysate Lane 8: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Histone H3 Antibody AW5292
    Western blot analysis of lysates from rat C6,Hela cell line (from left to right), using Histone H3 Antibody (Cat. #AW5292). AW5292 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P68431
Other Accession Q6LED0, P68433, P68432
Reactivity Human, Rat
Predicted Mouse
Host Mouse
Clonality Monoclonal
Calculated MW H=15;M=15;Rat=15 KDa
Isotype IgG1
Antigen Source HUMAN
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Antigen Region 1-156
Other Names Histone H31, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA
Dilution WB~~1:1000
Target/Specificity This Histone H3 antibody is generated from a mouse immunized with Histone H3 recombinant protein.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHistone H3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H3A
Synonyms H3FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.

BACKGROUND

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

REFERENCES

Zhong R.,et al.Nucleic Acids Res. 11:7409-7425(1983).
Marashi F.,et al.Biochem. Cell Biol. 64:277-289(1986).
Albig W.,et al.Genomics 10:940-948(1991).
Kardalinou E.,et al.J. Cell. Biochem. 52:375-383(1993).
Runge D.,et al.Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.

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