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MAGED1 Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - MAGED1 Antibody (Center) AW5278
    Western blot analysis of lysates from A2058,A375,Jurkat cell line (from left to right), using MAGED1 Antibody (Center)(Cat. #AW5278). AW5278 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9Y5V3
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=86,92 KDa
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 9500
Antigen Region 20-224
Other Names Melanoma-associated antigen D1, MAGE tumor antigen CCF, MAGE-D1 antigen, Neurotrophin receptor-interacting MAGE homolog, MAGED1, NRAGE
Dilution WB~~1:1000
Target/Specificity This MAGED1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 20-224 amino acids from the Central region of human MAGED1.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAGED1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAGED1
Synonyms NRAGE
Function Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=Expression shifts from the cytoplasm to the plasma membrane upon stimulation with NGF.
Tissue Location Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed
Research Areas

BACKGROUND

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds NGFR/p75NTR and antagonizes its association with NTRK1/TrkA, inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate TP53/p53 transcriptional activity and inhibit cell proliferation. Enhances TP53 phosphorylation and accumulation. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

REFERENCES

Salehi A.H.,et al.Neuron 27:279-288(2000).
Wen C.-J.,et al.FEBS Lett. 564:171-176(2004).
Chen Y.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Ross M.T.,et al.Nature 434:325-337(2005).
Pold M.,et al.Genomics 59:161-167(1999).

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