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PTGS1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PTGS1 Antibody (C-term) AW5276
    Western blot analysis of lysates from A431,K562 cell line (from left to right), using PTGS1 Antibody (C-term)(Cat. #AW5276). AW5276 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 3 - PTGS1 Antibody (C-term) AW5276
    Fluorescent image of Hela cell stained with PTGS1 Antibody (C-term)(Cat#AW5276).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTGS1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).PTGS1 immunoreactivity is localized to Golgi significantly.
  • 14 - PTGS1 Antibody (C-term) AW5276
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PTGS1 antibody (C-term) (Cat. #AW5276), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P23219
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=69,65 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5742
Antigen Region 571-599
Other Names PTGS1; COX1; Prostaglandin G/H synthase 1; Cyclooxygenase-1; Prostaglandin H2 synthase 1; Prostaglandin-endoperoxide synthase 1
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:10~50
Target/Specificity This PTGS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human PTGS1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTGS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTGS1
Synonyms COX1
Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
Cellular Location Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein
Research Areas

BACKGROUND

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis.

REFERENCES

Helmersson,J., Prostaglandins Leukot. Essent. Fatty Acids 80 (1), 51-56 (2009)Malkowski,M.G., Science 289 (5486), 1933-1937 (2000)

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