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EBP1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - EBP1 Antibody (Center) AW5271
    Western blot analysis of lysates from HepG2,mouse NIH/3T3 cell line (from left to right), using EBP1 Antibody (Center)(Cat. #AW5271). AW5271 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - EBP1 Antibody (Center) AW5271
    EBP1 Antibody (Center) (Cat. #AW5271) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - EBP1 Antibody (Center) AW5271
    EBP1 Antibody (Center) (Cat. #AW5271) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession Q9UQ80
Other Accession P50580
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=44;M=44 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5036
Antigen Region 228-255
Other Names PA2G4; EBP1; Proliferation-associated protein 2G4; Cell cycle protein p38-2G4 homolog; ErbB3-binding protein 1
Dilution WB~~ 1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human EBP1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEBP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PA2G4
Synonyms EBP1
Function May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).
Cellular Location Isoform 1: Cytoplasm Nucleus, nucleolus. Note=Tranlocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization
Tissue Location Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver

BACKGROUND

PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells.

REFERENCES

Zhang,Y., Mol. Cancer Ther. 7 (10), 3176-3186 (2008)
Zhang,Y., Cancer Lett. 265 (2), 298-306 (2008)
Okada,M., J. Biol. Chem. 282 (50), 36744-36754 (2007)

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