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HMGCS1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HMGCS1 Antibody (Center) AW5269
    Western blot analysis of lysates from mouse liver,rat liver,human liver tissue (from left to right), using HMGCS1 Antibody (Center)(Cat. #AW5269). AW5269 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - HMGCS1 Antibody (Center) AW5269
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - HMGCS1 Antibody (Center) AW5269
    Flow cytometric analysis of hela cells using HMGCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession Q01581
Other Accession P17425, Q8JZK9, P13704, P23228
Reactivity Human, Mouse, Rat
Predicted Hamster, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=57,M=58,Rat=57 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 3157
Antigen Region 290-317
Other Names HMGCS1; HMGCS; Hydroxymethylglutaryl-CoA synthase, cytoplasmic; 3-hydroxy-3-methylglutaryl coenzyme A synthase
Dilution WB~~ 1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-317 amino acids from the Central region of human HMGCS1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMGCS1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGCS1
Synonyms HMGCS
Function This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

HMGCS1 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

REFERENCES

Vock,C., Cell. Physiol. Biochem. 22 (5-6), 515-524 (2008)

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