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AMPD3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - AMPD3 Antibody (Center) AW5263
    Western blot analysis of lysates from human skeletal muscle,human kidney,mouse kidney tissue (from left to right), using AMPD3 Antibody (Center)(Cat. #AW5263). AW5263 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q01432
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=89,76,90,71 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 272
Antigen Region 325-356
Other Names AMPD3; AMP deaminase 3; AMP deaminase isoform E; Erythrocyte AMP deaminase
Dilution WB~~ 1:1000
Target/Specificity This AMPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-356 amino acids from the Central region of human AMPD3.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMPD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMPD3
Function AMP deaminase plays a critical role in energy metabolism.
Tissue Location Isoform 1 is the predominant form in skeletal muscle; Isoform 2 predominates in smooth muscle, non-muscle tissue, embryonic muscle and undifferentiated myoblasts; Isoform 3 is found in erythrocytes
Research Areas

BACKGROUND

AMPD3 is a member of the AMP deaminase gene family. This protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. The protein is the erythrocyte (E) isoforms, whereas other family members isoforms predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency.

REFERENCES

Mahnke-Zizelman D.K., Eddy R.Biochim. Biophys. Acta 1306:75-92(1996)
Yamada Y., Goto H., Wakamatsu N., Ogasawara N.Hum. Mutat. 17:78-78(2001)

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