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MDH1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - MDH1 Antibody (C-term) AW5245
    Western blot analysis of lysates from HL-60,THP-1 cell line (from left to right), using MDH1 Antibody (C-term)(Cat. #AW5245). AW5245 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - MDH1 Antibody (C-term) AW5245
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - MDH1 Antibody (C-term) AW5245
    MDH1 Antibody (C-term) (Cat. #AW5245) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P40925
Other Accession O88989, P11708, P14152, Q5ZME2, Q3T145
Reactivity Human
Predicted Mouse, Rat, Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=36,27,39;M=37;Rat=36 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 4190
Antigen Region 286-314
Other Names MDH1; MDHA; Malate dehydrogenase, cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Target/Specificity This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMDH1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MDH1
Synonyms MDHA
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

REFERENCES

Lee,S.M., et.al., Cell Death Differ. 16 (5), 738-748 (2009)

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