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ENOB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ENOB Antibody (Center) AW5243
    Western blot analysis of lysates from RD cell line,mouse skeletal muscle,rat skeletal muscle tissue lysate(from left to right), using ENOB Antibody (Center)(Cat. #AW5243). AW5243 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - ENOB Antibody (Center) AW5243
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ENOB Antibody (Center) AW5243
    ENOB Antibody (Center) (Cat.#AW5243) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P13929
Other Accession P15429, Q1KYT0, P21550, Q3ZC09
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=47,44,42;M=47;Rat=47 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 2027
Antigen Region 237-264
Other Names ENO3; Beta-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; Skeletal muscle enolase
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENOB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO3
Function Appears to have a function in striated muscle development and regeneration.
Cellular Location Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity)
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas

BACKGROUND

ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.

REFERENCES

Aurino,S., Acta Myol 27, 90-97 (2008)
Giallongo,A., Eur. J. Biochem. 214 (2), 367-374 (1993)

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