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YWHAG Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - YWHAG Antibody (N-term) AW5242
    Western blot analysis of lysates from Hela,A431 cell line,mouse brain tissue lysate(from left to right), using YWHAG Antibody (N-term)(Cat. #AW5242). AW5242 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - YWHAG Antibody (N-term) AW5242
    Formalin-fixed and paraffin-embedded human brain tissue reacted with YWHAG Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - YWHAG Antibody (N-term) AW5242
    YWHAG Antibody (N-term) (Cat. #AW5242) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P
Primary Accession P61981
Other Accession Q6NRY9, Q6PCG0, Q6PC29, P61983, P61982, Q5F3W6, P68252
Reactivity Human, Mouse
Predicted Rat, Zebrafish, Bovine, Chicken, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB21180
Calculated MW H=28,M=28,Rat=28 KDa
Additional info
Gene ID 7532
Other Names YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; 14-3-3 protein gamma, N-terminally processed
Target/Specificity This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human YWHAG.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsYWHAG Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YWHAG
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in brain, skeletal muscle, and heart.
Research Areas

BACKGROUND

YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

REFERENCES

Jagemann,L.R., et.al., J. Biol. Chem. 283 (25), 17450-17462 (2008)

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