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DHX9 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DHX9 Antibody (N-term) AW5241
    Western blot analysis of lysates from Jurkat,K562 cell line (from left to right), using DHX9 Antibody (N-term)(Cat. #AW5241). AW5241 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 3 - DHX9 Antibody (N-term) AW5241
    Fluorescent image of A549 cells stained with DHX9 Antibody (N-term)(Cat#AW5241). AW5241 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - DHX9 Antibody (N-term) AW5241
    Immunohistochemical analysis of paraffin-embedded H. colon section using DHX9 Antibody (N-term)(Cat#AW5241). AW5241 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - DHX9 Antibody (N-term) AW5241
    Immunohistochemical analysis of paraffin-embedded H. breast section using DHX9 Antibody (N-term)(Cat#AW5241). AW5241 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF
Primary Accession Q08211
Other Accession Q28141
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB44466
Calculated MW H=141;M=149,150 KDa
Additional info
Gene ID 1660
Other Names ATP-dependent RNA helicase A;DDX9; LKP; NDH2; ATP-dependent RNA helicase A; ATP-dependent RNA helicase A; DEAH box protein 9; ATP-dependent RNA helicase A; Leukophysin; ATP-dependent RNA helicase A; Nuclear DNA helicase II
Target/Specificity This DHX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DHX9.
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDHX9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DHX9
Synonyms DDX9, LKP, NDH2
Function Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD- mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. Positively regulates HIV-1 LTR-directed gene expression.
Cellular Location Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm

BACKGROUND

Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

REFERENCES

Lee C.-G., et al. J. Biol. Chem. 268:16822-16830(1993).
Abdelhaleem M.M., et al. J. Immunol. 156:2026-2035(1996).
Zhang S., et al. J. Biol. Chem. 272:11487-11494(1997).
Goshima N., et al. Nat. Methods 5:1011-1017(2008).
Gregory S.G., et al. Nature 441:315-321(2006).

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