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GPI Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - GPI Antibody (C-term) AW5240
    Western blot analysis of lysates from rat liver tissue lysate,Ramos cell line (from left to right), using GPI Antibody (C-term)(Cat. #AW5240). AW5240 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - GPI Antibody (C-term) AW5240
    GPI Antibody (C-term) (Cat. #AW5240) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB
Primary Accession P06744
Other Accession Q4R591
Reactivity Human, Rat
Predicted Mouse, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=63,64;M=63;Rat=63 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 2821
Antigen Region 445-473
Other Names GPI; Glucose-6-phosphate isomerase; Autocrine motility factor; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36
Dilution WB~~1:1000
FC~~1:10~50
Target/Specificity This GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPI
Function Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Cellular Location Cytoplasm. Secreted
Research Areas

BACKGROUND

GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

REFERENCES

Shih, W.L., et al. Cancer Lett. 290(2):223-237(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Araki, K., et al. J. Biol. Chem. 284(47):32305-32311(2009)
Tsutsumi, S., et al. Int. J. Oncol. 35(5):1117-1121(2009)
Funasaka, T., et al. Cancer Res. 69(13):5349-5356(2009)
Yanagawa, T., et al. J. Biol. Chem. 280(11):10419-10426(2005)
Haga, A., et al. Biochim. Biophys. Acta 1480 (1-2), 235-244 (2000)

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