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PGK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PGK1 Antibody (Center) AW5237
    Western blot analysis of lysates from Hela,PC-3,U-87 MG,A431,HL-60,Raji cell line (from left to right), using PGK1 Antibody (G132)(Cat. #AW5237). AW5237 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - PGK1 Antibody (Center) AW5237
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - PGK1 Antibody (Center) AW5237
    Confocal immunofluorescent analysis of PGK1 Antibody (Center)(Cat#AW5237) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 4 - PGK1 Antibody (Center) AW5237
    PGK1 Antibody (Center) (Cat. #AW5237) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 14 - PGK1 Antibody (Center) AW5237
    Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody (Center)(Cat#AW5237). AW5237 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, IF, WB
Primary Accession P00558
Other Accession P16617, P09411, Q60HD8
Reactivity Human
Predicted Mouse, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=45;M=45;Rat=45 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5230
Antigen Region 117-145
Other Names PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2
Dilution WB~~1:1000
IHC-P~~1:100
IF~~1:10~50
FC~~1:10~50
Target/Specificity This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGK1
Synonyms PGKA
Function In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

REFERENCES

Lay, A. J., et al. Nature 408: 869-873 (2000).

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