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|Application ||FC, IF, IHC-P, WB|
|Other Accession||P04961, P17918, P61258, P57761, Q9DEA3, Q3ZBW4|
|Reactivity||Human, Mouse, Rat|
|Predicted||Hamster, Monkey, Bovine, Chicken|
|Calculated MW||H=28;M=28;Rat=28 KDa|
|Other Names||PCNA; Proliferating cell nuclear antigen; Cyclin|
|Target/Specificity||This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PCNA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PCNA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.|
|Cellular Location||Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents|
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
Provided below are standard protocols that you may find useful for product applications.
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
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