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PCNA Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PCNA Antibody (Center) AW5234
    Western blot analysis of lysates from Hela,A431,Jurkat,mouse NIH/3T3,HepG2,Raji,rat PC-12 cell line (from left to right), using PCNA Antibody (Center)(Cat. #AW5234). AW5234 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - PCNA Antibody (Center) AW5234
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (Center) (Cat. #AW5234), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - PCNA Antibody (Center) AW5234
    Confocal immunofluorescent analysis of PCNA Antibody (Center)(Cat#AW5234) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
  • 4 - PCNA Antibody (Center) AW5234
    PCNA Antibody (Center) (Cat. #AW5234) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IF, IHC-P, WB
Primary Accession P12004
Other Accession P04961, P17918, P61258, P57761, Q9DEA3, Q3ZBW4
Reactivity Human, Mouse, Rat
Predicted Hamster, Monkey, Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=28;M=28;Rat=28 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 5111
Antigen Region 89-117
Other Names PCNA; Proliferating cell nuclear antigen; Cyclin
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
Target/Specificity This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PCNA.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCNA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCNA
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Cellular Location Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents
Research Areas
Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells.
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
29610564

BACKGROUND

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

REFERENCES

Wang,Y., J. Cell. Biochem. 106 (3), 409-413 (2009)
Maga,G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008)
Acharya,N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)

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