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UCHL1 Antibody (C-term)

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - UCHL1 Antibody (C-term) AW5233
    Western blot analysis of lysates from rat PC-12,U-87 MG,COS-7,mouse Neuro-2a,SH-SY5Y,Y79 cell line (from left to right), using UCHL1 Antibody (C-term)(Cat. #AW5233). AW5233 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P09936
Other Accession Q00981, Q6SEG5, Q9R0P9, Q60HC8, P23356
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=24;M=24;Rat=24 KDa
Isotype IgG1
Antigen Source HUMAN
Additional Information
Gene ID 7345
Antigen Region 185-214
Other Names UCHL1;Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1
Dilution WB~~1:1000
Target/Specificity This UCHL1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUCHL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UCHL1
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Cellular Location Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain
Tissue Location Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients
Research Areas

BACKGROUND

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

REFERENCES

Boudreaux D.A., et al. Proc. Natl. Acad. Sci. U.S.A. 107:9117-9122(2010).
Hillier L.W., et al. Nature 434:724-731(2005).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Day I.N.M., et al. Biochem. J. 268:521-524(1990).
Choi J., et al. J. Biol. Chem. 279:13256-13264(2004).

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