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STIP1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - STIP1 Antibody (Center) AW5230
    Western blot analysis of lysates from A549,A431 cell line (from left to right), using STIP1 Antibody (Center)(Cat. #AW5230). AW5230 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - STIP1 Antibody (Center) AW5230
    STIP1 Antibody (Center) (Cat. #AW5230) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 14 - STIP1 Antibody (Center) AW5230
    Formalin-fixed and paraffin-embedded human testis tissue reacted with STIP1 Antibody (Center) (Cat.# AW5230), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession P31948
Other Accession O35814, Q60864, O54981, Q3ZBZ8
Reactivity Human
Predicted Mouse, Rat, Hamster, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=63;M=63;Rat=63 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 10963
Antigen Region 269-297
Other Names STIP1; Stress-induced-phosphoprotein 1; Hsc70/Hsp90-organizing protein; Renal carcinoma antigen NY-REN-11; Transformation-sensitive protein IEF SSP 3521
Dilution WB~~1:1000
FC~~1:10~50
IHC-P~~1:50~100
Target/Specificity This STIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-297 amino acids from the Central region of human STIP1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTIP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STIP1
Function Acts as a co-chaperone for HSP90AA1 (PubMed:27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q60864}. Nucleus {ECO:0000250|UniProtKB:Q60864}
Research Areas

BACKGROUND

STIP1 mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).

REFERENCES

Onuoha,S.C., J. Mol. Biol. 379 (4), 732-744 (2008) Erlich,R.B., Glia 55 (16), 1690-1698 (2007)

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