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STIP1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - STIP1 Antibody (Center) AW5230
    Western blot analysis of lysates from A549,A431 cell line (from left to right), using STIP1 Antibody (Center)(Cat. #AW5230). AW5230 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - STIP1 Antibody (Center) AW5230
    STIP1 Antibody (Center) (Cat. #AW5230) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 14 - STIP1 Antibody (Center) AW5230
    Formalin-fixed and paraffin-embedded human testis tissue reacted with STIP1 Antibody (Center) (Cat.# AW5230), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC
Primary Accession P31948
Other Accession O35814, Q60864, O54981, Q3ZBZ8
Reactivity Human
Predicted Mouse, Rat, Hamster, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB17353
Calculated MW H=63;M=63;Rat=63 KDa
Additional info
Gene ID 10963
Other Names STIP1; Stress-induced-phosphoprotein 1; Hsc70/Hsp90-organizing protein; Renal carcinoma antigen NY-REN-11; Transformation-sensitive protein IEF SSP 3521
Target/Specificity This STIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-297 amino acids from the Central region of human STIP1.
Dilution WB~~1:1000
FC~~1:10~50
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTIP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STIP1
Function Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
Cellular Location Cytoplasm. Nucleus
Research Areas

BACKGROUND

STIP1 mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).

REFERENCES

Onuoha,S.C., J. Mol. Biol. 379 (4), 732-744 (2008) Erlich,R.B., Glia 55 (16), 1690-1698 (2007)

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