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CAPN1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - CAPN1 Antibody AW5228
    Western blot analysis of lysates from MCF-7,A549,A431 cell line (from left to right), using CAPN1 Antibody(Cat. #AW5228). AW5228 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - CAPN1 Antibody AW5228
    Flow cytometric analysis of Hela cells using CAPN1 Antibody(green, Cat#AW5228) compared to an isotype control of mouse IgG1(blue). AW5228 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - CAPN1 Antibody AW5228
    Immunohistochemical analysis of paraffin-embedded H. kidney section using CAPN1 Antibody(Cat#AW5228). AW5228 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession P07384
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=82 KDa
Isotype IgG1,k
Antigen Source HUMAN
Additional Information
Gene ID 823
Other Names Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, muCANP, CAPN1, CANPL1
Dilution WB~~1:1000
FC~~1:25
IHC-P~~1:25
Target/Specificity This CAPN1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN1.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAPN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAPN1
Synonyms CANPL1
Function Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Cellular Location Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding
Tissue Location Ubiquitous.
Research Areas

BACKGROUND

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

REFERENCES

Aoki K.,et al.FEBS Lett. 205:313-317(1986).
Sorimachi H.,et al.Biol. Chem. Hoppe-Seyler 371:171-176(1990).
Kim J.W.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
Melloni E.,et al.Biochem. Biophys. Res. Commun. 229:193-197(1996).
Michetti M.,et al.FEBS Lett. 392:11-15(1996).

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