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CYB5R3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CYB5R3 Antibody (Center) AW5217
    Western blot analysis of lysates from Hela,HepG2,A431 cell line,rat liver tissue lysate(from left to right), using CYB5R3(soluble form) Antibody (Center)(Cat. #AW5217). AW5217 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P00387
Other Accession P20070, P83686, Q9DCN2, Q60HG4, P07514
Reactivity Human, Rat
Predicted Mouse, Monkey, Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Clone Names RB49795
Calculated MW H=34,32,38;M=34,32;Rat=34,35,32 KDa
Additional info
Gene ID 1727
Other Names NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1
Target/Specificity This CYB5R3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-144 amino acids from the Central region of human CYB5R3
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5R3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5R3
Synonyms DIA1
Function Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Cellular Location Isoform 1: Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Mitochondrion outer membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Isoform 2 is expressed at late stages of erythroid maturation
Research Areas

BACKGROUND

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

REFERENCES

Tomatsu S.,et al.Gene 80:353-361(1989).
Voice M.W.,et al.Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
Yoon B.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Lan F.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.

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