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GSS Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - GSS Antibody (C-term) AW5216
    Western blot analysis of lysates from MDA-MB-453,Hela cell line (from left to right), using GSS Antibody (C-term)(Cat. #AW5216). AW5216 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - GSS Antibody (C-term) AW5216
    Immunohistochemical analysis of paraffin-embedded H. stomach section using GSS Antibody (C-term)(Cat#AW5216). AW5216 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P48637
Other Accession P46413, P51855, Q8HXX5, Q5EAC2
Reactivity Human
Predicted Mouse, Rat, Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW H=52;M=52;Rat=52 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 2937
Antigen Region 372-400
Other Names GSS; Glutathione synthetase; Glutathione synthase
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This GSS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the C-terminal region of human GSS.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSS
Research Areas

BACKGROUND

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSS functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione.

REFERENCES

Starr,J.M., et.al., Mech. Ageing Dev. 129 (12), 745-751 (2008)

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Anonymous
2016-08-24 09:20:44
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Species tested
Human,Mouse,Rat
Application tested
WB
Organization tested
Hela,U87,A549,LO2,293T,HT1080,SW480,Kindey(M),Kindey(R)
Barcode encoding
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose