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CLIC1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CLIC1 Antibody (Center) AW5215
    Western blot analysis of lysates from human placenta tissue lysate,HL-60,MCF-7,Raji,WiDr cell line (from left to right), using CLIC1 Antibody (Center)(Cat. #AW5215). AW5215 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O00299
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=27 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 1192
Antigen Region 136-166
Other Names CLIC1;Chloride intracellular channel protein 1
Dilution WB~~1:1000
Target/Specificity This CLIC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-166 amino acids from the Central region of human CLIC1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLIC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLIC1
Synonyms G6, NCC27
Function Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
Cellular Location Nucleus. Nucleus membrane; Single-pass membrane protein. Cytoplasm. Cell membrane; Single-pass membrane protein. Note=Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain
Tissue Location Expression is prominent in heart, placenta, liver, kidney and pancreas.
Research Areas

BACKGROUND

Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.

REFERENCES

Xie T., et al. Genome Res. 13:2621-2636(2003).
Shiina S., et al. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Valenzuela S.M., et al. J. Biol. Chem. 272:12575-12582(1997).
Noh Y.H., et al. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Chuang J.Z., et al. J. Neurosci. 19:2919-2928(1999).

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