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CALR Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CALR Antibody AW5211
    Western blot analysis of lysates from rat C6,Hela,SH-SY5Y cell line (from left to right), using CALR Antibody(Cat. #AW5211). AW5211 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - CALR Antibody AW5211
    CALR Antibody (Cat. #AW5211) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - CALR Antibody AW5211
    CALR Antibody (Cat. #AW5211) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P27797
Other Accession NP_004334.1
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=48;M=48;Rat=48 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 811
Antigen Region 1-437
Other Names CALR; CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; HACBP; grp60
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Target/Specificity This CALR Antibody is generated from rabbits immunized with a recombinant protein of human CALR.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCALR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CALR
Synonyms CRTC
Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Cellular Location Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen. Note=Also found in cell surface (T cells), cytosol and extracellular matrix Associated with the lytic granules in the cytolytic T-lymphocytes
Research Areas

BACKGROUND

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

REFERENCES

Du, X.L., et al. Oncogene 28(42):3714-3722(2009)
Sato, H., et al. Biochim. Biophys. Acta 1790(10):1198-1205(2009)
Abd Alla, J., et al. Biochem. Biophys. Res. Commun. 387(1):186-190(2009)

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