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UCHL1 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - UCHL1 Antibody AW5207
    Western blot analysis of lysates from U266,SH-SY5Y,mouse Neuro-2a cell line,mouse brain,rat brain tissue lysate,NCI-H460 cell line (from left to right), using UCHL1 Antibody(Cat. #AW5207). AW5207 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 3 - UCHL1 Antibody AW5207
    Fluorescent image of A549 cells stained with UCHL1 Antibody(Cat#AW5207). AW5207 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - UCHL1 Antibody AW5207
    Immunohistochemical analysis of paraffin-embedded H. kidney section using UCHL1(Cat#AW5207). AW5207 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P09936
Other Accession NP_004172.2
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Calculated MW H=25;M=25;Rat=25 KDa
Isotype IgG1,k
Antigen Source HUMAN
Additional Information
Gene ID 7345
Antigen Region 1-243
Other Names UCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:25
Target/Specificity This UCHL1 monoclonal antibody is generated from mouse immunized with UCHL1 recombinant protein.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUCHL1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UCHL1
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Cellular Location Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain
Tissue Location Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients
Research Areas

BACKGROUND

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.

REFERENCES

Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010)
Hussain, S., et al. Leukemia 24(9):1641-1655(2010)
Ma, Y., et al. J. Cell. Biochem. 110(6):1512-1519(2010)
Wu, Y.R., et al. Clin. Chim. Acta 411 (13-14), 955-958 (2010) :
Li, L., et al. Clin. Cancer Res. 16(11):2949-2958(2010)

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