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Beta-actin Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - Beta-actin Antibody AW5206
    All lanes : Anti-Beta-actin Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Human brain cell lysate Lane 4: Human kidney cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse kidney cell lysate Lane 7: PC-12 whole cell lysate Lane 8: Rat liver cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Beta-actin Antibody AW5206
    All lanes : Anti-Beta-actin Antibody at1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: human brain lysate Lane 4: human kidney lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: mouse kidney lysate Lane 7: PC12 whole cell lysate Lane 8: rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Beta-actin Antibody AW5206
    Western blot analysis of lysates from HepG2,mouse NIH/3T3,Hela cell line,mouse cerebellum,rat stomach tissue lysate,A431,THP-1,HL-60,rat PC-12 cell line (from left to right), using Beta-actin Antibody(Cat. #AW5206). AW5206 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P60709
Reactivity Human, Mouse, Rat
Predicted Bovine, Chicken, Xenopus
Host Mouse
Clonality Monoclonal
Clone Names 8H10D10
Calculated MW H=42;M=42;Rat=42 KDa
Additional info
Gene ID 60
Other Names ACTB; Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed
Target/Specificity ACTB recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeta-actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Research Areas

BACKGROUND

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

REFERENCES

Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.

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