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SSPN Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SSPN Antibody (N-term) AW5200
    Western blot analysis of lysates from HL-60,K562,MDA-MB-453 cell line (from left to right), using SSPN Antibody (N-term)(Cat. #AW5200). AW5200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q14714
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=27,14 KDa
Isotype Rabbit Ig
Antigen Source Human
Additional Information
Gene ID 8082
Antigen Region 6-34
Other Names SSPN;KRAG; Sarcospan; Sarcospan; K-ras oncogene-associated protein; Sarcospan; Kirsten-ras-associated protein
Dilution WB~~1:1000
Target/Specificity This SSPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human SSPN.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSSPN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SSPN
Synonyms KRAG
Function Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein Note=Also found in myotendinous junctions and in the postsynaptic membrane of neuromuscular junctions.
Tissue Location Isoform 1 is expressed exclusively in heart and skeletal muscle. Isoform 2 is expressed in heart, skeletal muscle, thymus, prostate, testis, ovary, small intestine, colon and spleen
Research Areas

BACKGROUND

Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.

REFERENCES

Crosbie R.H., et al. J. Biol. Chem. 272:31221-31224(1997).
Crosbie R.H., et al. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Wiemann S., et al. Genome Res. 11:422-435(2001).
Heighway J., et al. Genomics 35:207-214(1996).

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