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FUBP3 Antibody (N-term)

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - FUBP3 Antibody (N-term) AW5196
    Western blot analysis of lysates from Hela,U-251 MG cell line (from left to right), using FUBP3 Antibody (N-term)(Cat. #AW5196). AW5196 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 3 - FUBP3 Antibody (N-term) AW5196
    Fluorescent image of Hela cells stained with FUBP3 Antibody (N-term)(Cat#AW5196). AW5196 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF
Primary Accession Q96I24
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 1216CT820.155.150
Calculated MW H=62 KDa
Additional info
Gene ID 8939
Other Names FUBP3;FBP3; Far upstream element-binding protein 3
Target/Specificity Purified His-tagged FUBP3 protein was used to produced this monoclonal antibody.
Dilution WB~~1:1000
IF~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFUBP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FUBP3
Synonyms FBP3
Function May interact with single-stranded DNA from the far- upstream element (FUSE). May activate gene expression.
Cellular Location Nucleus.
Tissue Location Detected in a number of cell lines.

BACKGROUND

May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.

REFERENCES

Davis-Smyth T., et al. J. Biol. Chem. 271:31679-31687(1996).
Humphray S.J., et al. Nature 429:369-374(2004).
Bienvenut W.V., et al. Submitted (DEC-2008) to UniProtKB.
Lubec G., et al. Submitted (DEC-2008) to UniProtKB.
Matsuoka S., et al. Science 316:1160-1166(2007).

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