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CTSD Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - CTSD Antibody AW5191
    Western blot analysis of lysates from MCF-7,U-251 MG,HepG2 cell line (from left to right), using CTSD Antibody(Cat. #AW5191). AW5191 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - CTSD Antibody AW5191
    Immunohistochemical analysis of paraffin-embedded H. liver section using CTSD Antibody(Cat#AW5191). AW5191 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P07339
Reactivity Human
Predicted Mouse, Bovine
Host Mouse
Clonality Monoclonal
Calculated MW H=45 KDa
Isotype IgG1
Antigen Source Human
Additional Information
Gene ID 1509
Antigen Region 1-402
Other Names CTSD;CPSD; Cathepsin D; Cathepsin D light chain; Cathepsin D heavy chain
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity Purified His-tagged CTSD protein was used to produced this monoclonal antibody.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTSD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTSD
Synonyms CPSD
Function Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Cellular Location Lysosome. Melanosome. Secreted, extracellular space. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
Tissue Location Expressed in the aorta extrcellular space (at protein level).
Research Areas

BACKGROUND

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

REFERENCES

Burkard T.R., et al. BMC Syst. Biol. 5:17-17(2011).
Faust P.L., et al. Proc. Natl. Acad. Sci. U.S.A. 82:4910-4914(1985).
Westley B.R., et al. Nucleic Acids Res. 15:3773-3786(1987).
Redecker B., et al. DNA Cell Biol. 10:423-431(1991).
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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