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MGMT Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - MGMT Antibody AW5190
    Western blot analysis of lysates from MCF-7,Hela,Jurkat cell line (from left to right), using MGMT Antibody(Cat. #AW5190). AW5190 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 3 - MGMT Antibody AW5190
    Fluorescent image of Hela cells stained with MGMT Antibody(Cat#AW5190). AW5190 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - MGMT Antibody AW5190
    Immunohistochemical analysis of paraffin-embedded H. small intestine section using MGMT Antibody(Cat#AW5190). AW5190 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB
Primary Accession P16455
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=22 KDa
Isotype IgG1
Antigen Source Human
Additional Information
Gene ID 4255
Antigen Region 1-217
Other Names MGMT;Methylated-DNA--protein-cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA-alkyltransferase
Dilution WB~~1:1000
IF~~1:25
IHC-P~~1:25
Target/Specificity Purified His-tagged MGMT protein was used to produced this monoclonal antibody.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MGMT
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Cellular Location Nucleus.

BACKGROUND

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

REFERENCES

Edara S., et al. Carcinogenesis 16:1637-1642(1995).
Tano K., et al. Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
Rydberg B., et al. J. Biol. Chem. 265:9563-9569(1990).
Koike G., et al. J. Biol. Chem. 265:14754-14762(1990).
Hayakawa H., et al. J. Mol. Biol. 213:739-747(1990).

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