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ADH7 Antibody (C-Term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ADH7 Antibody (C-Term) AW5184
    Western blot analysis of lysates from SW480,HepG2 cell line (from left to right), using ADH7 Antibody (C-Term)(Cat. #AW5184). AW5184 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - ADH7 Antibody (C-Term) AW5184
    ADH7 Antibody (C-Term) (Cat. #AW5184) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH7 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ADH7 Antibody (C-Term) AW5184
    ADH7 Antibody (C-Term) (Cat. #AW5184) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - ADH7 Antibody (C-Term) AW5184
    Confocal immunofluorescent analysis of ADH7 Antibody (C-Term) (Cat. #AW5184) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, FC, IHC-P, WB
Primary Accession P40394
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=41,42;M=40;Rat=40 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 131
Antigen Region 318-346
Other Names ADH7; Alcohol dehydrogenase class 4 mu/sigma chain; Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Retinol dehydrogenase
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Target/Specificity This ADH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the C-terminal region of human ADH7.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADH7 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADH7
Function Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism.
Cellular Location Cytoplasm.
Tissue Location Preferentially expressed in stomach.
Research Areas

BACKGROUND

This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.

REFERENCES

Kedishvili, N.Y., et al. J. Biol. Chem. 270(8):3625-3630(1995)
Cheung, B., et al. Alcohol. Clin. Exp. Res. 19(1):185-186(1995)
Farres, J., et al. Eur. J. Biochem. 224(2):549-557(1994)
Pares, X., et al. FEBS Lett. 303(1):69-72(1992)

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